講師：Shuichi Onami教授(RIKEN Center for Biosystems Dynamics Research)
演題：『Imaging data-driven modeling of animal development and SSBD a database for imaging data-driven biology』
Quantitative data on spatiotemporal dynamics of biological systems provide crucial information for understanding the mechanism of biological systems. Combinations of live imaging and computational image-processing technologies have produced a large amount and a wide variety of quantitative dynamics data on biological systems. In this seminar, I will present our large collection of quantitative data on nuclear division dynamics in early C. elegans embryos. These data were obtained by combining four-dimensional microscopy and computational image processing under gene perturbations of each of all essential embryonic genes. Computational phenotype analysis identified 27,174 RNAi-induced phenotypic alteration from the fertilization up to the 8-cell stage. Clustering of phenotype profiles combined with GO analysis suggested early embryogenesis of C. elegans consists of 24 basic processes. We developed a method to elucidate causal relations of phenotypic expression by computing correlations between phenotypic characters, and another to infer genes involved in the causal relations of phenotype expression by computing outliers in the correlations. By combining these methods, we created a data-driven model of early C. elegans embryogenesis. I would also present our SSBD database (http://ssbd.qbic.riken.jp). SSBD stores and shares quantitative biological dynamics data and biological image data produced within Japan and beyond. SSBD provides infrastructure for imaging data-driven biology.